Region Of Interest After Effects Cs4 Serial Number
Region Of Interest After Effects Cs4 Serial Number >> https://tiurll.com/2ta7Yo
In your first use of tSEM, you have the option to use a larger field that may include a sample or a series of samples. For example, you may use it to image a small volume such as a single neuron in a region that has few sections imaged in your TM μ or EM data set. Typically, you would use a tSEM for this type of analysis to maintain the ability to measure in 3D (as previously discussed), and to avoid the potential artifacts seen with FIB-SEM and SBFSEM (unless you are working with thick sections). Although you can image a small volume of interest first with a tSEM, you might also just as easily do it with a conventional microscope using a serial section technique. Whether you are using a tSEM for this first analysis or not, you may find it useful to adopt a consistent order of image acquisition by using a tSEM. Once you acquire all of the volumes, you can continue to use the tSEM to acquire additional volumes on that same first sample (although you may need to adjust the area imaged on the tSEM depending on how much data you have acquired).
However, if you are using the tSEM to analyze a sample, you will first want to visualize the regions of interest on the screen as you acquire them. This will help you to locate the features of interest and to plan for your ultrastructural measurements, if you are carrying out quantitative ultrastructural analysis. In contrast to traditional analysis of ultrathin sections, your initial measurements may be on the order of 1-2 microns. This is still a useful size that will give you the ability to see structures with a resolution similar to that of light microscopy, but you may have to zoom in on a particular area to see all of the detail. The new elastic alignment tool in TrakEM2 [43] is much less sensitive to the section artifacts than prior alignment strategies, and provides accurate alignment across sections that would otherwise be distorted (Movie S1).
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